Supplementary data text 15 gener1

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Methods Fly stocks All crosses were carried out at 25oC under standard conditions. tor mutants were obtained by crossing tor with tor mutants. tor is the strongest constitutively activated tor mutation. pgc null mutant allele corresponds to the P-element insertion EY7794 (Martinho et al., 2004). pgc and tor double mutants stocks were obtained by recombination with tor or tor mutant alleles, and torpgc embryos are lied by transheterozygotic females carrying pgc tor/pgctor mutations. 6x[pgc] transgenic flies were obtained by injection of two rescuing constructs of pgc in the second chromosome. vastor double mutants were obtained by crossing 1574 and 2620 Bloomington stocks. His2av-GFP (R. Martinho/R. Lehmann) stocks were used for in vivo recordings.

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تاریخ انتشار 2009